The current research was conducted with the aim of constructing the type D personality interpretation bias questionnaire and its psychometric properties in college students. This research was conducted under the positivistic paradigm through a quantitative approach using exploratory and confirmatory factor analysis. The target population was the Persian Gulf University’s students of Bushehr, 277 of whom were selected by probability cluster sampling method from December2022 to February2023 (157 female and 118male). For measuring the variables, three questionnaires of type D personality (DS-14), well-being of Flourishing Scale (FS), and type D Personality Interpretation Biases (DIB) were used. Exploratory factor analysis was performed through SPSS-26 and confirmatory factor analysis was performed through AMOS-24. According to exploratory analysis, 11components with an eigenvalue higher than 1 were found. Each of the components included a number of items that had a factor load higher than 0.5. Guttman reliability and convergent and discriminant validity of the interpretation bias questionnaire were also confirmed. However, in confirmatory factor analysis, five components were removed and 6 components were remained with good fit indices. Therefore, because this questionnaire is a reliable and valid tool, it can be used by experts to measure the interpretation biases of type D personality people.